Virology Lab
Biosafety Level-2 Virology/Molecular Biology Laboratory
Biosafety Level-3 Laboratory
Immunology and Flow Cytometry Laboratory
Contact information: cprc.rcm+VL@upr.edu
RESEARCH INTERESTS:
- Immunogenicity and protective efficacy of multi-clade consensus ENV SHIV virus-like particle vaccine
- Cellular innate activation as a tactic to prevent HIV-1 transmission
- Molecular changes after serial DV infections in macaques
- Immunization of rhesus macaques with a dengue vaccine candidate based on a Venezuelan Equine Encephalitis (VEE) replicon vector
SERVICES PROVIDED:
- Real Time PCR
- Molecular cloning
- SDS-PAGE
- Western Blot
- ELISA
- PBMC's isolation and culture
- Cell proliferation
- Multiplex platform for cytokine detection by Luminex technology
- RNA extraction
- IFA experiments
- Immunophenotyping and dendritic cells determination
INSTRUMENTATION:
- For multiplex-cytokines analyses:
- Luminex100 system (Bio-Rad, Hercules, CA)
- For the Real-Time PCR experiments:
- iQ5 PCR machine
- To support the IFA experiments
- ELISA reader
- immunofluorescence microscope Olympus IX71
- For post-acquisition analysis:
- BD FACSAria flow cytometry unit
- detects up to 7 colors
- Summit software
- Other equipment:
- electrophoresis equipment
- blotting apparatus
- Beckman L8-M
- Beckman TJ6 centrifuge
- dual shakers temperature-controlled water bath
- three class II biological safety cabinets
- three double-door CO2 incubators
- four REVCO freezers
- four LN2 storage tanks
GRANT SUPPORT:
- 1R01AI084142-01
- CDC
- NIAID RFA-AI-06-033 (U01)
- NIH-RCE-NBC # AI57158
- NIH-RCE-NBC # AI57158
- P40 RR03640-23
- U24 RR 16021
- U24 RR 18108
